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KMID : 1007520190280020555
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Food Science and Biotechnology
2019 Volume.28 No. 2 p.555 ~ p.562
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Protective effects of chebulic acid from Terminalia chebula Retz. against t-BHP-induced oxidative stress by modulations of Nrf2 and its related enzymes in HepG2 cells
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Jung Hye-Lim
Yang Sung-Yong
Pyo Min-Cheol
Hong Chung-Oui
Nam Mi-Hyun
Lee Jin-Won
Lee Kwang-Won
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Abstract
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Although chebulic acid isolated from Terminalia chebular has diverse biological effects, its effects on the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of downstream genes have not been elucidated. The purpose of this research is to investigate the hepatoprotective mechanism of chebulic acid against oxidative stress produced by tert-butyl hydroperoxide (t-BHP) in liver cells. The treatment with chebulic acid attenuated cell death in t-BHP-induced HepG2 liver cells and increased intracellular glutathione content, upregulated the activity of heme oxygenase-1, and also increased the translocation of Nrf2 into the nucleus and Nrf2 target gene expression in a dose-dependent manner. The exposure of chebulic acid activated the phosphorylation of mitogen-activated protein kinases. The overall result is that chebulic acid has cytoprotective effect on t-BHP-induced hepatotoxicity in HepG2 cells through Nrf2-mediated antioxidant enzymes.
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KEYWORD
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Chebulic acid, Nuclear factor erythroid 2-related factor 2, Heme oxygenase-1, γ-Glutamate cysteine ligase, Mitogen-activated protein kinases
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